Veterinary Microbiology

AAU
AAU
 

AAU

BRIEF HISTORY

Established in 1964 as the then Bacteriology Department, at present the renamed Department of Veterinary Microbiology offers 6 different courses at undergraduate level, in addition to the internship training to the final year B.V.Sc. & A.H. students. The department offers 26 different courses at post-graduate level. So far 54 & 8 students have earned their M.V.Sc./M.Sc. & Ph.D. degrees respectively. The Department has 3 AAU funded Research Schemes viz., Central Disease Research Station (Bacterial), Diagnostic Centre for Mastitis and Research Centre for Viral Diseases. In addition, the department has ICAR funded Network Project on Haemorrhagic septicaemia. The department undertakes time-bound research projects under the activities of the above schemes, in addition to the diagnostic services being provided for various bacterial, viral and fungal diseases of animals and birds.

 

 Activity

  Teaching (U.G.Teaching)

Sr. No.

Name of Course

Course No.

Credits

1

General Veterinary Microbiology

VMC-211

1+1

2

Veterinary Immunology and Serology

VMC-221

1+1

3

Systematic Veterinary Bacteriology and Mycology

VMC-311

2+1

4

Systematic Veterinary Virology

VMC-321

2+1

5

Veterinary Clinical Biochemistry and Laboratory Diagnosis-II (Part of the course)

VLD-421

0+1

6

Animal Biotechnology (Part of the course)

VPB-321

2+1

 Teaching (P.G.Teaching)

Sr. No.

Name of Course

Course No.

Credits

  1.  

BACTERIOLOGY-I

VMC- 601

3+1

  1.  

BACTERIOLOGY-II

VMC-602

3+1

  1.  

VETERINARY MYCOLOGY

VMC-603

1+1

  1.  

GENERAL VIROLOGY

VMC-604

2+1

  1.  

SYSTEMIC ANIMAL VIROLOGY

VMC-605

3+1

  1.  

PRINCIPLES OF IMMUNOLOGY

VMC-606

2+1

  1.  

VACCINOLOGY

VMC-607

2+0

  1.  

DIAGNOSTICS OF INFECTIOUS DISEASES

VMC-608

1+2

  1.  

TECHNIQUES IN MICROBIOLOGY AND IMMUNOLOGY

VMC-609

0+3

  1.  

MASTER’S SEMINAR

VMC-691

1+0

  1.  

MASTER’S RESEARCH

VMC-699

20

  1.  

ADVANCES IN BACTERIOLOGY

VMC-701

2+1

  1.  

ADVANCES IN MYCOLOGY

VMC-702

2+1

  1.  

BACTERIAL GENETICS

VMC-703

2+1

  1.  

MICROBIAL TOXINS

VMC-704

2+1

  1.  

MOLECULAR DETERMINANTS OF BACTERIAL PATHOGENESIS

VMC-705

2+1

  1.  

ADVANCES IN VIROLOGY

VMC-706

2+1

  1.  

MOLECULAR AND GENETIC ASPECTS OF VIRAL PATHOGENESIS

VMC-707

2+1

  1.  

STRUCTURE FUNCTION RELATIONSHIP OF DNA AND RNA VIRUSES

VMC-708

3+0

  1.  

ONCOGENIC VIRUSES

VMC-709

2+0

  1.  

SLOW VIRAL INFECTIONS AND PRIONS

VMC-710

2+0

  1.  

MOLECULAR IMMUNOLOGY

VMC-711

2+1

  1.  

ADVANCES IN CELLULAR IMMUNOLOGY

VMC-712

2+1

  1.  

CYTOKINES AND IMMUNOMODULATORS

VMC-713

2+0

  1.  

ADVANCES IN VACCINOLOGY

VMC-714

2+0

  1.  

ADVANCES IN IMMUNODIAGNOSTICS

VMC-715

1+1

  1.  

MODERN IMMUNOTECHNOLOGY

VMC-716

1+2

  1.  

CURRENT TOPICS IN INFECTION AND IMMUNITY

VMC-717

3+0

  1.  

VETERINARY MICROBIAL BIOTECHNOLOGY

VMC-718

2+1

  1.  

SPECIAL PROBLEM

VMC-790

0+2

  1.  

DOCTORAL SEMINAR – I

VMC-791

1+0

  1.  

DOCTORAL SEMINAR – II

VMC-792

1+0

  1.  

DOCTORAL RESEARCH

VMC-799

45

 Number of P.G. students completing the degree programme (upto January 2012)

 

M.V.Sc.- 69                             Ph.D.- 09

  

Research

  Ongoing Research Projects

Sr. No.

Title

Start Date(period)

Agency

1

All India Network Project on Haemorrhagic septicaemia

November, 2000

(Plan)

ICAR, New Delhi

2

Central Disease Research Station (Bacterial)

1-12-1967

(Non-plan)

AAU, Anand

3

Research Centre for Viral Diseases

11-10-1979

(Non-plan)

AAU, Anand

4

Diagnostic Centre for Mastitis

2-1-1967

(Non-plan)

AAU, Anand

 Research Projects Completed

Sr. No.

Title

Duration

Agency

1

H.I testing in zoo and wild animals

1974-77

GAU

2

Infectious abortions in livestock at Gujarat

1974-77

ICAR

 Achievements/major research work done

Sr. No.

 

1

Veterinary Bacteriology and Mycology :

 

  1. Semen microbial assay. Effect of freezing of semen on bacterial load.
  2.  Identification of bacterial and fungal pathogens in reproductive tract infections of buffaloes and their antibiograms.
  3. Role of enterotoxigenic E.coli in neonatal diarrhoea and its antibiotic resistance pattern.
  4. Assessment of immune response to H.S. vaccine and effect of levamisole as an immunomodulator.
  5. Poultry Salmonella – Isolation, Characterization, Pathogenicity, virulence factors and determinants of antimicrobial drug resistance.
  6. Incidence of bacterial and mycotic bovine mastitis and comparison of various tests for detecting clinical and sub-clinical mastitis. Assessment of virulence factors and enterotoxigenicity of Staphylococcus aureus.
  7. Isolation and identification of Brucella from milk, semen and cases of reproductive disorders and abortions in animals.
  8. Isolation and identification of Listeria spp. from animals.
  9. Isolation, identification and characterization of Mycoplasma spp. from goats.
  10. Molecular characterization/gene detection/virulence factors of various bacterial pathogens viz. E.coli, Salmonella, Staphylococci, Streptococci, Listeria, Brucella, Pasteurella, Mycoplasma
  11. Rumen bacterial flora of goats.
  12. Metagenomic analysis of sub-clinical mastitis milk
  13. Whole genome sequencing of Pasteurella multocida

2

Veterinary Virology :

 

  1. Studies on incidence and/or characterization of various viral pathogens viz., Bovine rotavirus and BHV-1 of bovine; Blue tongue virus and PPR virus of sheep/goat, Canine parvovirus of dog and Infectious bursal disease virus, EDS-76 virus, Chicken anemia virus, Marek’s disease and Newcastle disease virus of poultry. Some of the above viruses were detected for the first time in the Gujarat State by this department.
  2. Many of the above viruses were cultivated in laboratory using tissue culture and/or egg embryo cultivation.
  3. Serotyping/genotyping of local virus isolates.
  4. Standardization of Polymerase chain reaction, Avidin-Biotin Enzyme linked immunosorbent assay (AB-ELISA) and Indirect-ELISA for diagnosis of Blue tongue virus. Standardization of Polymerase chain reaction and Dot-ELISA for detection of canine parvovirus.
  5. Immune response in birds against different vaccination regimes against Newcastle disease and Infectious bursal disease and interference by maternal antibodies.
  6. Nucleic acid profile of Bovine rotavirus, Blue tongue virus and Infectious

        bursal disease virus.

  1. Disease resistance pattern and virus detection studies on Infectious bursal disease in different breeds of experimentally infected chicken.
  2. Cell culture cultivation of infectious bursal disease virus, bovine rotavirus, BHV-1 and  canine parvovirus.
  3. Standardization and use of Polymerase chain reaction for diagnosis of

     Bluetongue virus, Canine parvovirus, IBD virus, PPR virus, BHV-1 and

     Chicken anemia virus.

j) Genetic characterization and variation studies on IBD virus, PPR virus,

    BHV-1 and Chicken anemia virus by RFLP and/or SSCP profiles.

k) Gene sequencing of F and N genes of PPR virus, gC and gD genes of BHV-

   1, Chicken anemia virus genes and VP2 gene of canine parvovirus.

3

Following recommendations from the Department have been approved in AGRESCO (Agriculture Research Council of the University).

  1. The maternal antibodies against Infectious bursal disease virus persisted upto about 20 days of age in chicks hatched from vaccinated dams. However, maternal antibodies were protective only till 15 days of age. Therefore, the chicks need to be vaccinated during this critical period.
  2. Immune response to inactivated vaccine against Infectious bursal disease at 7 days followed by live virus vaccine at 14 and 21 days of age was found to be satisfactory.
  3. Prevalence of Blue tongue virus in different species of animals reflects the importance of this agent, which demands exploring of all the possible avenues to understand this virus and the disease it causes.
  4. Serotyping studies in Blue tongue virus should form a base for any study relating to the vaccine production, as the prevalent serotypes used to be incorporated in the vaccine.
  5. Direct Fluorescent antibody technique can easily be adopted and is suitable to detect Blue tongue virus antigen in infected cultures.
  6. Role of Bluetongue virus in causing abortion in buffaloes should be further

Explored.

  1. Combinatorial PCR can be used for identification of pathogenic Brucella species using sets of primers.
  2. PCR based serotype detection of pathogenic Listeria monocytogenes isolates can be done using sets of primers.
  3. Based on in vitro sensitivity studies and PCR assay, use of sulfonamides in haemorrhagic septicaemia cases should be discouraged.
  4. New variant strain of canine parvovirus-2 i.e. CPV-2c detected in different canine population of the world is also circulating in the dogs of middle Gujarat region.

 Publications

 

Book (as co-author)                -           01

National Journal                      -           96

              International Journal               -           13

Review                                    -           05

Popular Articles (English)       -           13

Publications (Gujarati)            -           08

Gene séquences in GenBank  -           120

 

Training Programmes

 

               18 different refresher training programmes conducted for State Animal Husbandry and Dairy Co-operative officers (as on Jan 2012). Training Manuals prepared for each training programme and distributed.

 

Facilities Available

Sr. No.

Instruments/Facilities

1

Bacteriology Laboratory with culture room

2

Molecular and Postgraduate laboratory

3

Virology Tissue culture room

4

Laminar flow (2)

5

ELISA reader with automatic washer

6

Thermocyclers (3)

7

Nanodrop

8

Gel documentation system

9

Gel electrophoresis apparatus

10

CO2 incubator

11

Deep fridge and five refrigerators

12

Waterbath

13

Micro Oven

14

Centrifuges of different capacities

15

Water distillation plant

16

Four Computers with printers, Scanners, CD/DVD writers

 Award/Recognition

1

Hari Om Ashram Award to Dr.V.M.Jhala, for his work on “Detection of Rinderpest antibodies by Measles HI test”.

2

Sardar Krushi Awards to Dr.B.S.Chandel and Dr.H.N.Kher for their work on “Seroepidemiology, Isolation and Pathogenicity of Blue tongue virus”.

3

Chancellor’s Gold Medal for Best M.V.Sc. student to 3 PG students

4

Member of Executive Committee of IAVMI.

5

Ayurvet Research Award and Certificate of Excellence in Research for 2003 in the field of Poultry Science by Indian Poultry Science Association. 

6

Member of the Group Study Exchange (GSE) Programme of the Rotary International for the four week Professional and Cultural Exchange programme

7

Prof J. P. Trivedi Award sponsored by Shri Hari Om Ashram for the research contribution to Animal Science in the field of “Disease resistance pattern and virus detection studies on Infectious bursal disease (Gumboro) in different breeds of chicken” for the year 2003.

8

Best Teacher-BOLT Award at District level, sponsored by Air India-Gujarat Samachar for 2006.

9

Member of Academic Council of AAU for 3 years.

 Current area of research (Jan 2012)

  1. Molecular diagnosis and characterization of bacterial pathogens viz. Brucella, Mycoplasma, Pasteurella spp.
  2. Molecular diagnosis and characterization of viral pathogens viz. canine parvovirus.
  3. Whole genome sequencing of Pasteurella multocida.